Tuesday 13 – Friday 16 July 2010
Organisers: NJ Burroughs, T Bretschneider
Single cell monitoring techniques using fluorescent probes have revealed a significant spatial-temporal complexity underpinning even some of the simplest of cellular functions. For instance, spatial oscillations have been observed in the bacteria Escherichia coli and Bacillus subtilis, oscillations that are used to locate the centre of the cell in a Turing type instability [Howard04], temporal nucleus oscillations of transcription factors have been observed in signalling processes [Nelson et al 04], while exquisite regulation is required to produce the complex orchestration of many cellular functions, such as cell doubling (both DNA replication and physical division into two cells), cell signalling and cell movement. Further, these processes work within a context of error correction, self-organisation and noise. This theme will examine these issues, and include image analysis, spatial modelling, model inference and bioinformatics aspects. The theme will be highlighted over a month of activities, launched by an initial large workshop and followed by smaller workshops on particular topics. Topics will include: diffusion process inference (FRAP, photo activatable GFP, single particle tracking techniques), stochastic processes in networks (noise in transcription, translation), image analysis (registration, information extraction) and challenges of understanding regulation and orchestration of high level cellular functions such as cell movement, cell doubling, cell signalling, protein traffic, phagocytosis and differentiation. The topics are highly interdisciplinary, involving mathematical modelling and analysis, inverse problems, statistical inference, systems modelling and biophysics.
For more see FLUO2010